This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Large Scale Mutagenesis and Interference”.
1. Mutations can be introduced by ____________
a) Cloning
b) Recombination
c) Ligation
d) Gene targeting
View Answer
Answer: d
Explanation: Mutations can be introduced into predefined genes in vivo through a process termed as gene targeting which involves homologous recombination.
2. Gene-knockout is the generation of a null-gene.
a) True
b) False
View Answer
Answer: a
Explanation: When the aim is to inactivate the target gene completely and generate a null allele, the term gene knockout is often used.
3. Embryonic stem cells are __________
a) Totipotent
b) Pluripotent
c) Dead
d) Multipotent
View Answer
Answer: b
Explanation: Embryonic stem cells can be cultured like any established cell line, but they are derived from the very early mouse embryo and are therefore pluripotent.
4. The only organism in which systematic gene targeting has been achieved is ____________
a) Mouse
b) Drosophila
c) Fish
d) Yeast
View Answer
Answer: d
Explanation: The only organism in which systematic gene targeting has been achieved is the yeast Saccharomyces Cerevisiae. Its genome contains about 6000 open reading frames.
5. The EUROFAN project involves the use of _________ generated cassettes.
a) PCR
b) Ultracentrifugation
c) Electroporation
d) Cloning
View Answer
Answer: a
Explanation: The EUROFAN project involves the use of PCR-generated cassettes in which a selectable marker is placed between 50 basepair elements.
6. Genome-wide random mutagenesis is applicable to _________
a) Fish
b) Mammals
c) All organisms
d) Bacteria
View Answer
Answer: c
Explanation: Saturation mutagenesis has been used for many years to identify mutations affecting specific crucial biological processes.
7. Insertional mutagenesis facilitates __________
a) Cloning
b) PCR
c) Recombination
d) Centrifugation
View Answer
Answer: c
Explanation: Insertional mutagenesis leaves a DNA tag in the interrupted gene, which facilitates cloning and gene identification. It is the most popular mutagenesis strategy.
8. Ribozymes are _________ molecules.
a) Toxic
b) Large
c) DNA
d) Catalytic
View Answer
Answer: d
Explanation: Ribozymes are catalytic molecules that destroy targeted MRNAs. They carry out site-specific cleavage and ligation reactions.
Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.
To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.