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Biotechnology Questions and Answers

Internships in Biotechnology

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Sanfoundry located at Bangalore offers internships to deserving B.Tech/M.Tech Students in Biotechnology Branch. In Biotechnology internships offered by Sanfoundry, shortlisted interns will be working towards the creation of useful artifacts like questions and answers, tutorials, articles, real-world problems and solutions on Biotechnology. Moreover, every intern working on Biotechnology internship will focus on one specific subject under the System & Control branch. This will help the intern to develop an in-depth understanding of that particular subject in their branch.

Here’s the list of topics for Internship in “Biotechnology”.

  • Plant Genetic Engineering
  • Biochemistry
  • Fundamentals of Heat Transfer
  • Fundamentals of Mass Transfer
  • Chemical Engineering Thermodynamics
  • Chemical Process Calculations
  • Discrete Mathematics
  • Bioinformatics
  • Bioprocess Engineering
  • Molecular Biology
  • Cytogenetics
  • Vector Biology and Gene Manipulation
  • Solid State Chemistry
  • Human Anatomy and Physiology
  • IPR, Biosafety and Bioethics
  • Bioprocess Principles
  • Bioreactor Design and Analysis
  • Bioseparation Technology
  • Biostatistics, Computational Statistics and Geostatistics
  • DNA Typing, Proteomics and Beyond
  • Cell biology
  • Biotechnology and Applications
  • Animal Biotechnology
  • Chemical Reaction Engineering Analysis
  • Unit Operations
  • Downstream Processing
  • Engineering Graphics and Drawing
  • Entrepreneurship Development, Management and Apparel Entrepreneurship
  • Protein Engineering
  • Animal Physiology and Biotechnology
  • Pharmaceutical Biotechnology
  • Food Biotechnology
  • Personality Development
  • Bioinformatics, Microbial Genomics and Proteomics
  • Genetics
  • Bioinformatics Databases
  • Human Immunology
  • Immunotechnology
  • Industrial Biotechnology
  • Instrumentation and Process Control Theory
  • Biomathematics
  • Bioorganic Chemistry
  • Principles of Chemical Engineering
  • Advance Food Microbiology
  • Advance Molecular Biology
  • Momentum Transfer (MOT)
  • Nanobiotechnology
  • Topological Vector Spaces
  • Bioethics, IPR, IPRS, Patent Rights and Biodiversity
  • Bionanotechnology
  • Biophysics, Geophysics, Astrophysics, Brachytherapy Physics and Radiation Physics
  • Bioprocess Plant and Equipment Design
  • Energy Engineering
  • Microbiology
  • Clinical Science
  • Advance Structural Bioinformatics and Algorithms
  • Advance Bioinformatics
  • Biological Spectroscopy
  • Biomaterials Tissue interactions and Artificial Organs
  • BioMEMS and Systems On-Chip
  • Biomining
  • Biophysical Engineering
  • Bioprospecting
  • Therapeutic Nutrition
  • Biotechnology for Environmental Remediation
  • Bioterrorism
  • Brewing Science and Practice
  • Cancer Biology
  • Computation of Biological Molecules
  • Biomolecular Modeling and Simulation
  • Developmental Biology
  • Distillates and Fermentation Technology
  • Drug and Pharmaceutical Biotechnology
  • Biobusiness Management
  • Environmental Biotechnology
  • Computer Applications in Environmental Engineering
  • Biodiversity and informatics, Taxonomy and Conservation
  • Enzyme Science and Engineering
  • Enzymology and Enzyme Technology
  • Fermentation Technology and Applications
  • Biotechnology in Food Fermentation
  • Structural Biology
  • Food Fermentation Technology
  • Virology
  • Advance Mechanical Operations in Food Processing
  • inducible Gene Expression in Plants
  • Industrial Enzymology
  • Macromolecular interaction
  • Biotechnology of Plant Metabolites
  • Medical Diagnostics
  • Metabolic Engineering
  • Molecular Farming
  • Molecular Modelling and Drug Design
  • Structure and Functions of Biomolecules
  • Molecular Pathogenesis
  • Perl Programming
  • Tissue Engineering
  • Introduction to Perl Programming
  • Stem Cell in Health Care
  • Energy Furnaces
  • Biocatalysis
  • Engineering Mathematics
  • Engineering Physics
  • Engineering Chemistry
  • Engineering Mechanics
  • Engineering Drawing
  • Environmental Science and Engineering
  • Basic Electrical Engineering
  • Elements of Civil Engineering
  • Algorithms and Data Structures – 1
  • Algorithms and Data Structures – 2
  • Applied Chemistry
  • Basic Electronics Engineering
  • Advance Engineering Mathematics
  • Partial Differential Equations and Transform Theorems
  • Computer Aided Engineering Drawing
  • Multivariable Calculus and Differential Equations
  • Probability and Statistics
  • Differential and Difference Equations
  • Applications of Differential and Difference Equations
  • Elements of Mechanical Engineering
    • Sanfoundry is looking for Interns who are passionate about their field of study and like core subjects in Biotechnology. Every intern contributes to Sanfoundry’s Global learning project during their internship and is Mentored and Guided by our Founder and CTO. If you are interested to contribute and apply, here’s full detail of Sanfoundry’s Internship Program.


    Sanfoundry Global Education & Learning Series – Biotechnology Internships!




    Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @
    LinkedIn | Facebook | Twitter



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    Gene Manipulation Questions and Answers – Gene Inactivation and Inhibition

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    This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Gene Inactivation and Inhibition”.

    1. Many gene inactivation strategies don’t require gene modification.
    a) True
    b) False
    View Answer

    Answer: a
    Explanation: Many strategies for gene inactivation do not require the direct modification of the target gene. Traditional gene transfer strategies add new genetic information to the genome.

    2. Antisense RNA blocks the activity of _______
    a) DNA
    b) mRNA
    c) CDNA
    d) RNA
    View Answer

    Answer: b
    Explanation: Antisense RNA blocks the activity of mRNA in a stoichiometric manner. Antisense RNA has the opposite sense to mRNA.

    3. Presence of antisense and complementary sense RNA can lead to the formation of _______
    a) Mutation
    b) Duplex
    c) Carcinogens
    d) Protein
    View Answer

    Answer: b
    Explanation: The presence of complementary sense and antisense RNA molecules in the same cells can lead to the formation of a stable duplex.

    4. Antisense RNA can be used in eukaryotes.
    a) True
    b) False
    View Answer

    Answer: b
    Explanation: Antisense RNA is used as a natural mechanism to regulate gene expression in a number of prokaryote systems and to a lesser extent in other organisms.

    5. Which type of inhibition can be achieved using antisense RNA?
    a) Stable
    b) Unstable
    c) Transient
    d) Integrative
    View Answer

    Answer: c
    Explanation: Transient inhibition of particular genes can be achieved by directly introducing antisense RNA or antisense oligonucleotides into cells.

    6. What is MBP?
    a) Inducer
    b) Protein
    c) Promoter
    d) Gene
    View Answer

    Answer: b
    Explanation: An expression cassette was constructed in which the mouse myelin basic protein CDNA was inverted with respect to the promoter.

    7. Which gene is targeted in transgenic tomato plant?
    a) Mg
    b) Pg
    c) Gfp
    d) Mbp
    View Answer

    Answer: b
    Explanation: Smith in 1988 generated transgenic tomato plants carrying an antisense construct targeting the endogenous polygalacturonase gene.

    8. Level of inhibition does not depend on ________ of antisense RNA.
    a) Size
    b) Temperature
    c) Ph
    d) Range
    View Answer

    Answer: a
    Explanation: The level of inhibition apparently does not depend on the size of the antisense RNA or the part of the endogenous gene to which it is complimentary.

    Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.

    To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.



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    Gene Manipulation Questions and Answers – Large Scale Mutagenesis and Interference

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    This set of Gene Manipulation Multiple Choice Questions & Answers (MCQs) focuses on “Large Scale Mutagenesis and Interference”.

    1. Mutations can be introduced by ____________
    a) Cloning
    b) Recombination
    c) Ligation
    d) Gene targeting
    View Answer

    Answer: d
    Explanation: Mutations can be introduced into predefined genes in vivo through a process termed as gene targeting which involves homologous recombination.

    2. Gene-knockout is the generation of a null-gene.
    a) True
    b) False
    View Answer

    Answer: a
    Explanation: When the aim is to inactivate the target gene completely and generate a null allele, the term gene knockout is often used.

    3. Embryonic stem cells are __________
    a) Totipotent
    b) Pluripotent
    c) Dead
    d) Multipotent
    View Answer

    Answer: b
    Explanation: Embryonic stem cells can be cultured like any established cell line, but they are derived from the very early mouse embryo and are therefore pluripotent.

    4. The only organism in which systematic gene targeting has been achieved is ____________
    a) Mouse
    b) Drosophila
    c) Fish
    d) Yeast
    View Answer

    Answer: d
    Explanation: The only organism in which systematic gene targeting has been achieved is the yeast Saccharomyces Cerevisiae. Its genome contains about 6000 open reading frames.

    5. The EUROFAN project involves the use of _________ generated cassettes.
    a) PCR
    b) Ultracentrifugation
    c) Electroporation
    d) Cloning
    View Answer

    Answer: a
    Explanation: The EUROFAN project involves the use of PCR-generated cassettes in which a selectable marker is placed between 50 basepair elements.

    6. Genome-wide random mutagenesis is applicable to _________
    a) Fish
    b) Mammals
    c) All organisms
    d) Bacteria
    View Answer

    Answer: c
    Explanation: Saturation mutagenesis has been used for many years to identify mutations affecting specific crucial biological processes.

    7. Insertional mutagenesis facilitates __________
    a) Cloning
    b) PCR
    c) Recombination
    d) Centrifugation
    View Answer

    Answer: c
    Explanation: Insertional mutagenesis leaves a DNA tag in the interrupted gene, which facilitates cloning and gene identification. It is the most popular mutagenesis strategy.

    8. Ribozymes are _________ molecules.
    a) Toxic
    b) Large
    c) DNA
    d) Catalytic
    View Answer

    Answer: d
    Explanation: Ribozymes are catalytic molecules that destroy targeted MRNAs. They carry out site-specific cleavage and ligation reactions.

    Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation.

    To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers.



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